Disaccharidase Determination

Small Bowel Biopsy
Lactase, sucrase, maltase, and palatinase (in lieu of isomaltase) activities can be determined from an intestinal biopsy weighing approximately 5 mg wet weight. The enzyme activities are determined in small bowel biopsies taken via endoscope by the referring clinical physician.
  • Includes

    Lactase, Sucrase, Maltase, Palatinase

  • Methodology

    Spectrophotometry (SP)

  • Assay Category

    This test was developed and its analytical performance characteristics have been determined by Joli Diagnostic Inc. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

  • Shipping Instructions

    Keep sample frozen and stored at -20C to -70C until it can be shipped on dry ice.
    Package according to regulations regarding shipment of infectious material and pack with enough dry ice to last 48 hours.
    Ship overnight by appropriate courier (i.e. UPS/ FedEx).

  • Shipping Container

    Insulated carton with foam shipper / dry ice / tightly sealed with tape

  • Transport Temperature

    Frozen on dry ice.

  • Turn-Around Time

    Results same day as receipt of specimen.

  • Results

    Results will be faxed and mailed upon assay completion. Bill will be sent to the institution from which the sample was sent. Should the sample not be of sufficient size to do a total analysis, then $47.97 will be charged for each enzyme analyzed.

  • Preferred Specimen(s)

    5 mg frozen small bowel biopsy

  • Minimum Volume

    2 mg

  • Collection Instructions

    Approximately 5 mg of small bowel should be collected through endoscopy for the complete enzyme analysis.
    The tissue should be placed on the wall of a small, tightly capped, plastic tube appropriately labeled with the patient name, date of birth, ID number, date specimen collected and referring physician name. Freeze and store at -20C to -70C until it can be shipped.

  • Specimen Stability

    Room temperature: Unacceptable
    Refrigerated: Unacceptable
    Frozen: 31 days

  • Reject Criteria

    Samples immersed in a colorless fluid (i.e. formalin, saline, etc.) Samples placed on gauze, filter paper, or any other type of support or embedding media/material.

  • Related Documents

    Sample Report