Pancreatic Enzyme Determination

Small Bowel Biopsy
Determination of pancreatic enzyme activities (amylase, chymotrypsin, lipase, and trypsin) in duodenal secretions, usually in conjunction with a secretin stimulation test performed by the referring physician. Pancreatic enzyme supplementation therapy should be discontinued prior to sample collection.
  • Includes

    Amylase, Typsin, Chymotrypsin, Protein, Lipase

  • Methodology

    Amylase, trypsin, chymotrypsin and protein are quantitatively determined by spectrophotometry. Lipase is determined by pH titration.

  • Assay Category

    This test was developed and its analytical performance characteristics have been determined by Joli Diagnostic Inc. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

  • Shipping Instructions

    Keep sample frozen and stored at -20C to -70C until it can be shipped.
    Package according to regulations regarding shipment of infectious material and pack with enough dry ice to last 48 hours.
    Ship overnight by appropriate courier (i.e. UPS/ FedEx).

  • Shipping Container

    Small, tightly capped plastic tube.

  • Transport Temperature

    Frozen and ship on dry ice.

  • Turn-Around Time

    Test performed weekly.

  • Results

    Results will be faxed and mailed immediately upon assay completion.
    Bill will be sent to the institution from which the sample was sent.

  • Preferred Specimen(s)

    Approximately 1 mL of duodenal fluid is necessary to perform the total enzyme analysis.

  • Minimum Volume


  • Collection Instructions

    Approximately 1 mL of duodenal fluid is necessary to perform the total enzyme analysis. The sample can be a single fluid or part of a secretin stimulation test.
    The fluid must have a pH of 6.0 or greater at the time of collection. A lower pH suggests gastric contamination, which will inactivate the enzyme activities.
    The duodenal fluid should be placed in a small, tightly capped plastic tube appropriately labeled with the patient name, date of birth, ID number, date specimen collected and name of referring physician.

  • Specimen Stability

    Room temperature: Unacceptable
    Refrigerated: Unacceptable
    Frozen: 31 days

  • Reject Criteria

    Samples immersed in a colorless fluid (i.e., water, formalin, saline, etc.) Samples placed on graze, filter paper, or any other type of support or embedding media/material.

  • Related Documents

    Sample Report